INTRODUCTION:

Fennel is a generally spread normal plant which is found in practically all regions of the planet. Organically it is known as Foeniculum vulgare and has a place with the family Apiaceae. It is utilized as a zest and food as well as a famous people medication for different diseases. The significance that it holds today had been perceived a long time back in China, India and Egypt. In Fennel, the guideline fixing which seems, by all accounts, to be a natural oil was refined out and named allicin (C₆H₁₀S₂₀) in 1944, which contains allylpropyl-disulphide, diallyldisulphide and a few other sulfur compounds. The concentration of natural oil is 0.3 to 0.4%. It has strong cell reinforcement, anticarcinogenic, antimutagenic, antiparasitic, antifungal, antiparasitic, and antiatherosclerotic and immunomodulatory reactions^1-3.

Formaldehyde, a water solvent lackluster gas, is the individual from aldehyde family where a few mixtures like acetaldehyde, malondialdehyde, acrolein, benzaldehyde, vanillin and citral are available. It's unadulterated structure is aggravation and fetor. Its strong state is called paraformaldehyde and it can transform into its vaporous state, formaldehyde, in room temperature. It was first utilized in the modern field in 1868. Formalin and urea formaldehyde are the most generally utilized types of formaldehyde. WHO proclaimed the items that contain formaldehyde in a nitty gritty way in 1985. As per this declaration the items containing formaldehyde are as per the following: cements and pastes, deodrants, cleansers, restorative merchandise, cleansers, channels and synthetics, manures, paints and particularly white papers, explosives, formica, elastic, compressed wood, finish, stain, eraser, plastic, defensive covering utilized in keeping food sources and in the creation and tanning of cowhide and fur, water conditioners and material products. On the adverse consequences of formaldehyde, a few epidemiological examinations have been directed which have analyzed the relationship among formaldehyde and malignant growth risk in people. These adverse consequences can be arranged in two headings like aggravation (intense and persistent) and carcinogenic.^4-6

Electrolytes are minerals that are found in body tissues and blood as disintegrated salts. The equilibrium of the electrolytes in our body is fundamental for typical capability of our cells and our organs. Sodium (Na+) and potassium (K+) are normal electrolytes that are estimated with the assistance of blood testing. Electrolytes assist with moving supplements into and squanders out of the body's cells, sustain a solid water equilibrium and assist with settling the body's corrosive base equilibrium. The general equilibrium of these synthetics is a sign of the useful prosperity of a few essential body functions.^7-9

MATERIALS AND METHODS:

Experimental Animal:

24 male Wistar pale skinned person rodents, Rattus norvegicus (Berkenhout) were chosen for the current review.

Maintenance and Feeding of Experimental Animal:

Solid pale skinned person rodents of practically equivalent size and weight ranges were kept in the polypropylene confines estimating 45x27x15cm at the temperature 25±0.5ºC and relative dampness 50±2.5%. The rodents were taken care of with standard lab pellet food and water not obligatory all through the examination.¹⁰

Experimental Compounds:

In the current review, Fennel oil was utilized as a cell reinforcement. 1ml/100g b wt. Fennel oil was given to each rodent/day for 30 days. Fennel is a rich wellspring of sulfur compounds. Formaldehyde, a receptive lackluster aldehyde gas with impactful scent framed by deficient burning of hydrocarbons was chosen for the current review. The rodents in the trial sets were presented to 6 ppm formaldehyde fumes in openness chamber for one hour day to day for 30 days.¹¹

Experimental Protocol:

The trial pale skinned person rodents were gathered into three sets arbitrarily as control set 1, exploratory set 2 and exploratory set 3. Control Set 1The rodents of control set 1 were not presented to formaldehyde fumes and no supplementation was given. Trial Set 2 the rodents of exploratory set 2 were presented to 6ppm formaldehyde fumes for one hour day to day for 30 days. Trial Set 3 the rodents of exploratory set 3 were presented to 6ppm formaldehyde fumes for one hour alongside supplementation of cancer prevention agent Fennel oil for 30 days.^12-14

Exposure to Formaldehyde Vapors:

The exploratory rodents were kept in a confined little openness chamber for one hour inside their enclosures and were evaluated by the trial convention.^15-18

Statistical Calculations:

For each biochemical boundaries at least 10 repeats were finished and the outcomes were genuinely broke down by student's't' test.^19-21

Mean (x): The mean was determined by the accompanying equation:

Where,

åx = Amount of individual perception, N= No. of perceptions.

Standard Deviation (SD):

The standard deviation was determined by the accompanying recipe:

Where,

= The amount of squares of all deviation.

Standard Error of Mean (S.Em):

Standard mistake of mean was determined by the accompanying equation:

Where,

S.D. = Standard deviation, N= No. of observations.

Student ‘t’ Test:

To begin with, 'S' was determined by the accompanying recipe: -

Where,

S = standard deviation of the distinction between two examples, X and Y these are factors, X and Y are the mean of two factors, N1 and N2 are the no. of perceptions of the two factors x and y, Then, at that point, the 't' was determined by the accompanying equation:-

Degree of Freedom (d.f.):

The level of opportunity was determined by the accompanying recipe:-

d.f. = N₁+N₂-2

The 't' esteem were meant by the Fisher's equation importance test. The likelihood 'p' for getting 't' an incentive for a given level of not entirely set in stone for a given level of opportunity.

Level of Confidence:

The measurable trial of the likelihood of committing type I of blunder at specific level called degree of certainty and limit the possibilities committing the sort II mistake. The degree of trust in the likelihood at committing type I blunder. In our examination the p esteem was 0.05.

In our own choice the possibilities happening the sort II blunder are around 5% it implies 95% of the perceptions will unquestionably be correct, so in this analysis the hypothesized worth of p was 0.05 and 'p' values was connoted as follows:

P> 0.05

or non-significant

P= 0.05

P<0.05

or significant*

P= 0.02

P<0.01

or Highly significant**

P= 0.01

P= 0.001

or Very highly significant ***

P<0.001

RESULTS:

In the current review, changes in the degree of serum electrolytes viz., sodium (Na+) and potassium (K+) have been seen in Wistar pale skinned person rodents Rattus norvegicus (Berkenhout). Defensive job of cell reinforcement Fennel oil has been seen in formaldehyde uncovered rodents.

Serum sodium particle (Na+) level in Wistar rodents diminished altogether after organization of Fennel oil which got expanded profoundly fundamentally after openness to formaldehyde vapors (Table-I). While serum potassium (K+) particle level expanded profoundly fundamentally after supplementation of Fennel oil in contrast with an exceptionally huge decline in the serum potassium particle level after formaldehyde openness (Table-II).

Table 1: The upsides of serum sodium (Na+) particle (mEq/l) focus in Wistar rodents after openness to formaldehyde fumes and Fennel oil supplementation.

Sets	Exposure	Mean ± S.Em.	Significant Difference from Corresponding
Sets	Exposure	Mean ± S.Em.	Set 1	Set 2
Control Set 1	Unexposed	121.0 ± 11.38	–	–
Experimental Set 2	Exposed to formaldehyde vapors	180.7 ± 5.88	↑***	–
Experimental Set 3	Formaldehyde vapors + Fennel oil (antioxidant)	135.8 ± 6.55	↑***	↓**

S.Em. = Standard Error of Mean, ↑ Increase, ** Significant, ↓ Decrease, *** Highly significant

Table 2: The upsides of serum potassium (K+) particle (mEq/l) fixation in wistar rodents after openness to formaldehyde fumes and Fennel oil supplementation.

Sets	Exposure	Mean ± S.Em.	Significant Difference From Corresponding
Sets	Exposure	Mean ± S.Em.	Set 1	Set 2
Control set 1	Unexposed	4.8 ± 0.145	–	–
Experimental Set 2	Exposed to formaldehyde vapours	3.8 ± 0.487	↓***	–
Experimental Set 3	Formaldehyde vapours + Fennel oil (antioxidant)	4.6 ± 1.254	↑*	↑***

S.Em. = Standard Error of Mean, ↑ Increase, * Non Significant, ** Significant, ↓ Decrease, *** Highly significant

DISCUSSION:

In the current review, a huge expansion in the sodium (Na+) particle fixation while decline in potassium (K+) particle focus after formaldehyde openness is reminiscent of hypernatremic and hypokalemic impacts separately when contrasted with the control set of rodents. Hypernatremia might create because of overactivity of the adrenal cortex. Sodium is essentially an extracellular particle and is very familiar in corrosive base²². Potassium then again is prevalently an intracellular particle. The fixation slope is kept up with chiefly by the Na+/K+ siphon. Potassium is discharged through kidney and discharge is expanded by unreasonable admission of sodium particles. An excessive amount of discharge of potassium by the kidneys prompts hypokalemia^23-25. The cationic and anionic fixation aggravation in pale skinned person rodents might be the consequence of poisonous activity of formaldehyde fumes bringing about sickly hypoxia in rodents, which animates respiratory focus and upset the corrosive base equilibrium in the body. The low oxygen content of the air animates breath and oxyhemoglobin frees oxygen more effectively within the sight of electrolytes than in their nonappearance^26-28. Supporting discoveries are additionally given by past explores who expressed that corrosive base equilibrium gets upset because of retention of air poisons while some other review noticed frail condition in bunnies after openness to formaldehyde fume. One of the review depicted that formaldehyde can cause extreme tissue injury by delivering receptive oxygen species.^29-32

In the continuous review, the supplementation of Fennel oil showed a huge reduction in serum sodium (Na+) particle level while an increment is found in potassium (K+) particle level separately. Naturalproducts like Fennel and its rejuvenating balm showed its significance to battle against different physiological dangers including oxidative pressure, invulnerable brokenness and disease rebellion. Fennel extricates have exhibited hypotensive movement in tolerably hypertensive subjects. Alliin and allicin, sulfur containing compounds are viewed as the most trademark constituent of Fennel. The amino corrosive alliin is the most delegate sulfur compound in new Fennel and is switched over completely to allicin by allinase catalyst when Fennel is squashed 33-35. The cell reinforcement capability of Fennel oil is credited to the presence of organo-sulfur intensifies that adjust glutathione and GST movement. The adequacy of Fennel has been connected with its powerful cell reinforcement properties 36-37. The parts of Fennel oil will rummage free extremists and safeguard films from injury and keeps up with cell trustworthiness 38-40. From the review we can at last say that Fennel had a strong cell reinforcement framework and limited intracellular oxidative pressure. As per the current review that watery Fennel remove goes about as a cancer prevention agent by rummaging Receptive Oxygen Species (ROS) and upgrading cell reinforcement catalysts.

CONCLUSION:

Fennel oil supplementation has been shown to decrease serum sodium ion levels and increase potassium ion levels. Fennel and its essential oil are important for fighting against physiological threats like oxidative stress, immune dysfunction, and cancer. Fennel extracts have hypotensive activity in moderately hypertensive subjects. Fennel's sulfur-containing compounds, alliin and allicin, have antioxidant potential and are effective in scavenging free radicals and maintaining cell integrity. Fennel extract has been shown to mitigate toxicity induced by acrylamide exposure and has potent anti-inflammatory and antioxidant effects, making it an effective means of mitigating complications in ageing diseases.

REFERENCES:

1. Burange PJ, Tawar MG, Bairagi RA, Malviya VR, Sahu VK, Shewatkar SN, Sawarkar RA, Mamurkar RR. Synthesis of silver nanoparticles by using Aloe vera and Thuja orientalis leaves extract and their biological activity: a comprehensive review. Bulletin of the National Research Centre. 2021 Dec; 45:1-3.

2. Malviya V. Preparation and Evaluation of Emulsomes as a Drug Delivery System for Bifonazole. Indian Journal of Pharmaceutical Education and Research. 2021 Jan 1; 55(1):86-94.

3. Malviya V, Ladhake V, Gajbiye K, Satao J, Tawar M. Design and characterization of phase transition system of zolmitriptan hydrochloride for nasal drug delivery system. International Journal of Pharmaceutical Sciences and Nanotechnology. 2020 May 31; 13(3):4942-51.

4. Malviya V, Pande S. Development and Evaluation of Fast dissolving Film of Fluoxetine hydrochloride. Research Journal of Pharmacy and Technology. 2021; 14(10):5345-50.

5. Malviya V. Design and Characterization of Thermosensitive Mucoadhesive Nasal Gel for Meclizine Hydrochloride: Thermosensitive Nala Gel of Meclizine HCL. International Journal of Pharmaceutical Sciences and Nanotechnology. 2022 Feb 28; 15(1):5782-93.

6. Malviya, Vedanshu, Mukund Tawar, Prashant Burange, and Rahul Jodh. "A brief review on resveratrol." (2022): 157-162.

7. Malviya V, Burange P, Thakur Y, Tawar M. Enhancement of Solubility and Dissolution Rate of Atazanavir Sulfate by Nanocrystallization. Indian Journal of Pharmaceutical Education and Research. 2021 Jul 1; 55(3):S672-80.

8. Malviya V, Arya A, Burange P, Gajbhiye K, Rathod G, Tawar M. To evaluate the cardioprotective effect of hydroalcoholic extract of Matricaria chamomilla linn in isoproterenol induced myocardial infarction in wistar rats. Research Journal of Pharmacy and Technology. 2022; 15(9):3887-92.

9. Malviya V, Tawar M, Burange P, Bairagi R. Preparation and Characterization of Gastroreten-tive Sustained Release In-situ Gel of Lafutidine. International Journal of Pharmaceutical Sciences and Nanotechnology. 2022 Dec 12; 15(6):6216-28.

10. Mahmood RT, Shamim A, Ahsan F, Parveen S. Foeniculum vulgare, Solanum nigrum and Cichorium intybus: A Collectanea of Pharmacological and Clinical uses. Research Journal of Pharmacy and Technology. 2021; 14(1):555-61.

11. Malviya V. Investigation of In-Vitro Antidiabetic Study, Antioxidant Activity and Anthelminthic Property of Various Extracts of Bitter Cumin Seeds: Antidiabetic Study, Antioxidant Activity, and Anthelminthic Property of Bitter Cumin Seeds. International Journal of Pharmaceutical Sciences and Nanotechnology. 2023 Jul 31; 16(4):6855-74.

12. Boddupalli BM, Ramani R, Mung’oma M, Muraga M, Anisetti RN, Nemala A. Common Edible Essential oils with strong potential against Breast Cancer and their Mechanisms. Research Journal of Pharmacy and Technology. 2023; 16(1):477-80.

13. Rufaida TM, Shamim A, Ahsan F, Parveen S, Shariq M. Foeniculum vulgare, Solanum nigrum and Cichorium intybus: A Collectanea of Pharmacological and Clinical uses. In Vitro. 30(31): 32.

14. Singh S, Dhande SR, Aggarwal SM, Suryawanshi A, Kadam V. In vitro antioxidant activity of 70% methanolic extracts of roots of Hemidesmus indicus. Research Journal of Pharmacy and Technology. 2012; 5(9):1241-5.

15. Malviya V. Investigation of In-Vitro Antidiabetic Study, Antioxidant Activity and Anthelminthic Property of Various Extracts of Bitter Cumin Seeds: Antidiabetic Study, Antioxidant Activity, and Anthelminthic Property of Bitter Cumin Seeds. International Journal of Pharmaceutical Sciences and Nanotechnology. 2023 Jul 31; 16(4):6855-74.

16. Malviya V. Box-Behnken Modeling Served for the Development and Optimization of Nanoparticles Loaded with Perindopril and Erbumine. Asian Journal of Pharmaceutics. 2023 Sep 15; 17(03).

17. Himalian R, Singh MP. A Comparative account on Antioxidant activities, Total phenolic and Flavonoid contents of Punica granatum, Carica papaya, Foeniculum vulgare, Trigonella foenum-graecum, and Urtica dioica: An in vitro Evaluation. Research Journal of Pharmacy and Technology. 2022; 15(3):1175-83.

18. Malviya V, Thakur Y, Gudadhe SS, Tawar M. Formulation and evaluation of natural gum based fast dissolving tablet of Meclizine hydrochloride by using 3 factorial design 2. Asian Journal of Pharmacy and Pharmacology. 2020; 6(2):94-100.

19. Malviya VR, Pande SD. Road CKN. Preparation ad Evaluation of Zolmitriptan Hydrochloride Lozenge. J Pharma Res. 2019; 8(8):624-9.

20. Malviya VR, Pande SD, Bobade NN. Preparation and evaluation of sustained release beads of zolmitriptan hydrochloride. Research Journal of Pharmacy and Technology. 2019; 12(12):5972-6.

21. Malviya VR, Tawar MG. Preparation and evaluation of oral dispersible strips of teneligliptin hydrobromide for treatment of diabetes mellitus. International Journal of Pharmaceutical Sciences and Nanotechnology. 2020 Jan 31; 13(1):4745-52.

22. Malviya V, Manekar S. Design, development and evaluation of aceclofenac and curcumin agglomerates by Crystallo Co-Agglomeration technique. Research Journal of Pharmacy and Technology. 2021; 14(3):1535-41.

23. Malviya V, Pande S. Design and Characterization of pH Dependent Phase Transition System of Almotriptan Malate for Nasal Drug Delivery System by Employing Factorial Design. Indian J of Pharmaceutical Education and Research. 2024; 58(1): 76-90.

24. Zeeshan A, Akram H, Qasim S, Naseer A, Nazar F, Rafique O. The Healing Touch of Foeniculum vulgare Mill.(Fennel): A Review on Its Medicinal Value and Health Benefits. Journal of Health and Rehabilitation Research. 2023 Dec 24; 3(2):793-800.

25. Rafieian F, Amani R, Rezaei A, Karaça AC, Jafari SM. Exploring fennel (Foeniculum vulgare): Composition, functional properties, potential health benefits, and safety. Critical Reviews in Food Science and Nutrition. 2023 Feb 2:1-8.

26. Portincasa P, Bonfrate L, Scribano ML, Kohn A, Caporaso N, Festi D, Campanale MC, Di Rienzo T, Guarino M, Taddia M, Fogli MV. Curcumin and Fennel Essential Oil Improve Symptoms and Quality of Life in Patients with Irritable Bowel Syndrome. Journal of Gastrointestinal & Liver Diseases. 2016 Jun 1; 25(2).

27. Saber JI, Eshra DH. Using fennel seeds and their oil as a preservative and functional food to produce some food and drink products to alleviate cough symptoms. Alexandria Science Exchange Journal. 2019 Sep 30; 40(July-September):406-14.

28. Ansari M, Kargar S, Eslami MA, Falahati R, Albenzio M, Caroprese M, Zamiri MJ, Kanani M. Potential benefits of early-life supplementation of liquid feed with fennel (Foeniculum vulgare) seeds or oregano (Origanum vulgare) leaves on growth, health, and blood metabolites in Holstein dairy calves. Journal of Dairy Science. 2022 Aug 1; 105(8):6639-53.

29. Mohamad RH, El-Bastawesy AM, Abdel-Monem MG, Noor AM, Al-Mehdar HA, Sharawy SM, El-Merzabani MM. Antioxidant and anticarcinogenic effects of methanolic extract and volatile oil of fennel seeds (Foeniculum vulgare). Journal of Medicinal Food. 2011 Sep 1; 14(9):986-1001.

30. Rathore SS, Saxena SN, Singh B. Potential health benefits of major seed spices. Int J Seed Spices. 2013 Jul; 3(2):1-2.

31. Elghazaly NA, Radwan EH, Zaatout HH, Elghazaly MM, El din Allam N. Beneficial effects of fennel (Foeniculum vulgare) in treating obesity in rats. Journal of Obesity Management. 2019 Jan 17; 1(2):16-33.

32. Khadivzadeh T, Najafi MN, Kargarfard L, Ghazanfarpour M, Dizavandi FR, Khorsand I. Effect of fennel on the health status of menopausal women: a systematic and meta-analysis. Journal of Menopausal Medicine. 2018 Apr 1; 24(1):67-74.

33. Saddiqi HA, Iqbal Z. Usage and significance of fennel (Foeniculum vulgare Mill.) seeds in Eastern medicine. Nuts and Seeds in Health and Disease Prevention. 2011 Jan 1 (pp. 461-467). Academic Press.

34. Aziz AH. Potential of making labneh fortified with different types of basil and fennel of healthy benefits. Journal of Food and Dairy Sciences. 2011 Aug 1; 2(8):387-98.

35. Kian FR, Bekhradi R, Rahimi R, Golzareh P, Mehran A. Evaluating the effect of fennel soft capsules on the quality of life and its different aspects in menopausal women: a randomized clinical trial. Nursing Practice Today. 2017 Jul 18; 4(2):87-95.

36. Syed FQ, Mirza MB, Elkady AI, Hakeem KR, Alkarim S. An Insight of Multitudinous and Inveterate Pharmacological Applications of Foeniculum vulgare (Fennel). Plant and Human Health, Volume 3: Pharmacology and Therapeutic Uses. 2019:231-54.

37. Noreen S, Tufail T, Badar Ul Ain H, Awuchi CG. Pharmacological, nutraceutical, functional and therapeutic properties of fennel (foeniculum vulgare). International Journal of Food Properties. 2023 Dec 31; 26(1):915-27.

38. Kaur J, Kaur S, Mahajan A. Herbal medicines: possible risks and benefits. Am J Phytomed Clin Ther. 2013; 1(2):226-39.

39. Santos J, Alfaro MC, Trujillo-Cayado LA, Calero N, Muñoz J. Encapsulation of β-carotene in emulgels-based delivery systems formulated with sweet fennel oil. LWT. 2019 Feb 1; 100:189-95.

40. Khan RU, Fatima A, Naz S, Ragni M, Tarricone S, Tufarelli V. Perspective, opportunities and challenges in using fennel (Foeniculum vulgare) in poultry health and production as an eco-friendly alternative to antibiotics: a review. Antibiotics. 2022 Feb 20; 11(2):278.

Received on 02.01.2024 Modified on 27.04.2024

Res. J. Pharma. Dosage Forms and Tech.2024; 16(3):205-209.

DOI: 10.52711/0975-4377.2024.00032

Statistical Calculations:

For each biochemical boundaries at least 10 repeats were finished and the outcomes were genuinely broke down by student's't' test.19-21

For each biochemical boundaries at least 10 repeats were finished and the outcomes were genuinely broke down by student's't' test.^19-21